Substratum Compliance Regulates Human Trabecular Meshwork Cell Behaviors and Response to Latrunculin B
نویسندگان
چکیده
منابع مشابه
Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells.
PURPOSE To determine the impact of substratum stiffness and latrunculin-B (Lat-B), on the expression of several matrix proteins that are associated with glaucoma. METHODS Human trabecular meshwork (HTM) cells were cultured on hydrogels possessing stiffness values mimicking those found in normal (5 kPa) and glaucomatous meshworks (75 kPa), or tissue culture polystyrene (TCP; >1 GPa). Cells wer...
متن کاملEffects of latrunculin-B on outflow facility and trabecular meshwork structure in human eyes.
PURPOSE To determine the effect of the F-actin-disrupting agent latrunculin-B on aqueous outflow facility and trabecular meshwork architecture in human eyes. METHODS After baseline facility measurement in human eye bank eyes (n = 9 pairs), one eye of each pair received anterior chamber exchange and continued perfusion with medium containing 1 microM latrunculin-B. Contralateral eyes were trea...
متن کاملLatrunculin B effects on trabecular meshwork and corneal endothelial morphology in monkeys.
To determine the mechanism of latrunculin B (LAT-B)-induced decrease in outflow resistance and the effect of LAT-B on the cornea, structural changes of the trabecular meshwork (TM) and the corneal endothelium following LAT-B were studied in the live monkey eye. LAT-B (0.5 microM) and vehicle were administered by anterior chamber exchange and infusion with cationized and non-cationized gold solu...
متن کاملEffect of latrunculin-A on morphology and actin-associated adhesions of cultured human trabecular meshwork cells.
PURPOSE Determine the effects of the actin cytoskeleton disrupting compound latrunculin-A (LAT-A) on morphology, cytoskeleton, and cellular adhesions of cultured human trabecular meshwork (HTM) cells. METHODS HTM cells were cultured to high confluence with endothelial-like morphology and treated with LAT-A at different doses and duration. Topography of living cells was evaluated by videomicro...
متن کاملHuman trabecular meshwork cell volume regulation.
The volume of certain subpopulations of trabecular meshwork (TM) cells may modify outflow resistance of aqueous humor, thereby altering intraocular pressure. This study examines the contribution that Na+/H+, Cl-/HCO exchange, and K+-Cl- efflux mechanisms have on the volume of TM cells. Volume, Cl- currents, and intracellular Ca2+ activity of cultured human TM cells were studied with calcein flu...
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ژورنال
عنوان ژورنال: Investigative Opthalmology & Visual Science
سال: 2011
ISSN: 1552-5783
DOI: 10.1167/iovs.11-7857